| abstract |
Some aspects of the disclosure include a guide nucleotide sequence programmable DNA binding protein domain (eg, a nuclease inactive variant of Cas9 such as dCas9), an optional linker, and a recombinase catalytic domain (eg, a tyrosine recombinase catalytic domain or a serine recombinase catalytic domain). For example, a Gin recombinase catalytic domain). This fusion protein can recombine a DNA site containing a minimal recombinase core site flanked by guide RNA specific sequences. The present disclosure represents a step independent of endogenous cellular mechanisms or states to programmable scarless genome editing in unmodified cells. |