| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2523-303
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-156
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-125
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-143
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-519 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-574
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate |
2017-06-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2019-08-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2019522476-A |
| titleOfInvention |
Detection of nucleic acid mutations using magnetic bead actuation and detection |
| abstract |
The present invention relates to a method of distinguishing mutations, single nucleotide polymorphisms or other variants in a target nucleic acid sequence in a sample from a wild type sequence. The method comprises the steps of surface binding magnetic beads via sandwich hybridization in which a bead-bound probe hybridizes with one end of the target nucleic acid and a surface-bound probe hybridizes with multiple ends of the same target nucleic acid, and magnetic and / or temperature. Applying stringency to the hybridization by determining the amount of magnetic beads remaining attached to the surface, and determining the amount of magnetic beads remaining attached to the surface by the target nucleic acid. And correlating with the presence or absence of mutations in SNP variants or other variants. |
| priorityDate |
2016-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |