| abstract |
In various embodiments, disclosed herein is an assay for measuring generated thrombin (TG) in a blood sample, the assay incubating the blood sample with TF, FIXa, and CaCl2. And measuring TG in a blood sample. Also disclosed herein is an assay for determining the risk of bleeding in a subject, the assay obtaining a blood sample from the subject; adding TF and / or FIXa to the blood sample Determining the amount of factor VIII coagulation factor (FVIII: C) in the blood sample; and (a) a mild bleeding risk in the subject if the amount of FVIII: C in the sample is> 5 IU / dL. And (b) if the amount of FVIII: C in the sample is 1 to 5 IU / dL, then moderate bleeding risk in the subject, and (c) the amount of FVIII: C in the subject is <1 IU / dL. If this is the case, then this includes determining the risk of severe bleeding in the subject. |