| abstract |
The subject matter of the present disclosure provides compositions and methods including the improvement of CRISPR systems (e.g., CRISPR Related (Cas) 9 (Crisps-Cas 9, non-Cas 9 CRISPR systems). Such compositions include modifications to the H1 promoter region, Addition of 5 'UTR modification, different orthologous sequence of H1 promoter, novel compact bidirectional promoter sequence with activity of both pol II and pol III, addition of Kozak consensus sequence, termination sequence, conditional pol II / pol It may include the addition of III bidirectional promoter expression, the addition of donor template sequences to correct mutations, or a combination thereof Other aspects of the invention are directed to Cas9 via post-transcriptional cell cycle regulatory fusion. Modifications, nucleases bind without DNA cleavage Engineered partially target site to cut, self-regulatory sites, and a half-life about N-terminal modification for modulating. |