| abstract |
The present invention is a functional inhibitor of water, at least one carbon source, one or more vitamins, one or more salts, one or more fatty acids, one or more buffer components, selenium, acid sphingomyelinase A synthetic medium of known chemical composition for eukaryotic cell culture, comprising at least one substance of the group of FIASMA) and at least one polypeptide of the TGF-β superfamily having the ability to inhibit stem cell differentiation The use thereof in the culture of pluripotent stem cells, a cell culture system comprising human pluripotent stem cells and a synthetic medium of known chemical composition, and a kit for the growth and / or maintenance of human pluripotent stem cells. |