abstract |
The present invention uses a yeast strain engineered to have lactate dehydrogenase (LDH) activity and optionally reduced or knocked out pyruvate decarboxylase (PDC) activity, using glucose as a carbon source A method for producing lactic acid in recombinant yeast cell culture, wherein the yeast is cultured in a medium of pH 5-7, a first seed fermentation step for producing biomass, and then the yeast The method is provided, comprising a second production fermentation step using biomass derived from seed fermentation for producing lactic acid, and culturing the L. agar in a low pH medium. |