| abstract |
Described herein are methods of producing a number of protein products from blood-based materials, including alpha 1 -proteinase inhibitors, gamma globulin, albumin, and other proteins. The method of the invention includes the steps of salt fractionation, chromatography, ultrafiltration, diafiltration, solvent-surfactant treatment, and sterile filtration. Advantageously, the method of the present invention is simple and produces alpha 1 -proteinase inhibitor, gamma globulin, albumin and other proteins in high yield. The order of process steps can be selected to obtain a large number of products from various intermediates such as supernatants, pastes, chromatography flow-throughs, and chromatography washes. |