http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019509050-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2770-36143 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-85 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-76 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-69 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-86 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-40 |
| filingDate | 2017-03-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-04-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2019509050-A |
| titleOfInvention | RNA replicon for versatile and efficient gene expression |
| abstract | The invention encompasses RNA replicons that can be replicated by alphavirus-derived replicases. RNA replicons contain sequence elements that are required for replication by replicase, but these sequence elements do not encode proteins or fragments thereof, such as alphavirus nonstructural proteins or fragments thereof. Therefore, in the RNA replicon according to the present invention, the sequence element required for replication by the replicase and the protein coding region are not coupled. According to the present invention, uncoupling is achieved by removal of at least one start codon compared to native alphavirus genomic RNA. In particular, the RNA replicon comprises a 5 'replication recognition sequence, wherein the 5' replication recognition sequence is characterized by the removal of at least one start codon compared to the native alphavirus 5 'replication recognition sequence. Alphavirus-derived replicases can be encoded by open reading frames on RNA replicons or on separate RNA molecules. The present invention allows efficient and safe expression of a protein of interest in a cell or organism, but is not associated with undesired production of fragments of alphavirus nonstructural proteins. In addition to in vitro and in vivo protein production methods, medical uses are also provided herein. [Selection] Figure 1 |
| priorityDate | 2016-03-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1639.