| abstract |
Disclosed herein are human or humanized tissues and organs suitable for transplantation. Host animal genetic editing provides a niche for complementing lost genetic information by donor stem cells. Editing the host genome to knockout or weaken genes involved in the growth and / or differentiation of the target organ, and injecting donor stem cells into animals at the embryonic stage, and lost genetics regarding organ growth and development Complementing information. The result is a chimeric animal in which the complemented tissue (human / humanized organ) is matched to the donor's genotype and phenotype. Such organs can be created in one generation, and stem cells can be harvested and created from the patient's own body. As disclosed herein, it is possible to do this by editing multiple genes simultaneously in cells or embryos that generate a “niche” for complementary tissue. In vertebrate cells or embryos, multiple genes can be targeted for editing using targeted nucleases and homologous recombination repair (HDR) templates. [Selection figure] None |