| abstract |
The present invention relates to a deoxynucleoside tri comprising a 3′-O position capped by a group comprising methylene disulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of the deoxynucleoside. Methods, compositions, mixtures and kits utilizing phosphate are provided. In one embodiment, the present invention contemplates nucleotide analogs having a reversible protecting group comprising methylene disulfide and an oxymethylene disulfide linker cleavable between the label and the nucleobase. Such compounds offer new possibilities for future sequencing techniques including but not limited to synthetic sequencing. |