| abstract |
The present invention provides a reference data set for pluripotent stem cells for predicting the functionality and suitability of pluripotent stem cell lines, and a method, system and kit for producing a scorecard. A scorecard comprising using at least two stem cell assays selected from epigenetic profiling, differentiation assays, and gene expression assays to predict the functionality and suitability of pluripotent stem cell lines. Of preparation methods, systems and kits. Scorecard reference data can be compared to pluripotent stem cell line data to effectively and accurately predict the utility of pluripotent stem cells for a given application, eg, therapeutic use, drug screening, and toxicity assays, And specific features of pluripotent stem cell lines can be identified to determine their suitability for differentiation into cell lineages. [Selected figure] Figure 25 |