http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019075993-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_faf522b8b83eded745c73018576219ba |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-04 |
| filingDate | 2017-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_912d31b64760190e57daac70a46a0dd5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_77b85c77f9f8bb3d823cec63dd842548 |
| publicationDate | 2019-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2019075993-A |
| titleOfInvention | Method for three-dimensional culture of cells, cell structure, method for producing cell structure and method for carrying cells |
| abstract | The present invention provides a three-dimensional culture method of cells in which cell death due to excessive aggregation of microcarriers and cells is suppressed. A method for three-dimensional culture of cells comprises: mixing a first solution containing polysaccharides, a microcarrier, and adherent cells, and adding a second solution containing a coagulant to the polysaccharides. A gelation step of gelling the polysaccharide, a third solution containing a polyamino acid is added, and a mixture containing the microcarriers, the adherent cells and the polysaccharide in gel form is added with the polyamino acid. A first coating step of coating, a fourth solution of adding a solubilizing agent for the gelled polysaccharide, and removing the polysaccharide, the microcarrier coated with the polyamino acid, and And C. culturing the mixture containing the adherent cells. 【Selection chart】 None |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/KR-20230127997-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2023085441-A1 http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022145420-A1 |
| priorityDate | 2017-10-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 377.