| abstract |
An artificial skin replicated so as to be close to actual pigmented skin, and a method for producing the same. A method for producing artificial skin exhibiting pigmentation using only keratinocytes, comprising the step of mixing fibroblasts and collagen to form a dermal replication layer, wherein the keratinization layer is formed on the dermal replication layer. Culturing after applying the cells, culturing the dermal replication layer in a first medium that is a keratinocyte medium, and mixing the dermal replication layer cultured in the first medium with DMEM medium and F12 medium A method for producing artificial skin, comprising culturing in a second medium. [Selection] Figure 1 |