| abstract |
The present disclosure provides a plurality of nucleic acid sequences comprising a plurality of variants of a reference sequence. The present disclosure further provides plasmids, cells, methods, and kits comprising the same. In one embodiment, a nucleic acid molecule comprising a plurality of reference variants is disclosed. In other embodiments, mixtures or combinations of nucleic acid molecules comprising variants of the reference sequence are disclosed. In certain embodiments, the nucleic acid molecule or mixture of nucleic acid molecules comprises one or more variants that are present at high or low frequency. In certain embodiments, the present disclosure provides a control reagent comprising a plurality of nucleic acid molecules. [Selection figure] None |