http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018532413-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-07006 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2320-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2330-51 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-205 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1247 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-00 |
| filingDate | 2016-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-11-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2018532413-A |
| titleOfInvention | Enhancement and expression of protein expression |
| abstract | The present disclosure relates generally to modified Gram-positive bacteria cells that produce increased amounts of one or more proteins of interest and modified Gram-positive bacteria cells that have increased gene competency. Accordingly, certain embodiments of the present disclosure relate to modified Gram-positive bacterial cells that express increased amounts of the target protein relative to unmodified (parent) Gram-positive bacterial cells that express the same protein of interest, wherein the modified bacterial cell Contains at least one mutation in the rpoC gene encoding a mutant RNA-polymerase (RNAP) β′-subunit polypeptide. In certain embodiments, the rpoC gene encoding the mutant β′-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpoC gene encoding the mutant β′-subunit polypeptide is contained on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure provides at least one nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and a substitution of aspartate to glycine at position 796 of SEQ ID NO: 8. A competent Bacillus host cell comprising a copy, wherein a polynucleotide encoding an rpoC polypeptide is non-competent prior to introduction of the first nucleic acid construct and a Bacillus host cell Is exogenous. [Selection] Figure 1 |
| priorityDate | 2015-10-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 721.