http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018526030-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1048 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-115 |
| filingDate | 2016-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2018526030-A |
| titleOfInvention | Method for aptamer selection for unbound targets |
| abstract | The present invention relates to a method for the selection of aptamers that do not require target or oligonucleotide library immobilization. The method comprises: (i) mixing an oligonucleotide selection library comprising a random region sandwiched between primer recognition sites with a blocker oligonucleotide that anneals to the primer recognition site; (ii) a blocked selection library Is exposed to an immobilization field containing random oligonucleotides and removes unbound selection library oligonucleotides; (iii) recovers selection library oligonucleotides bound to immobilization fields And (iv) exposing the selection library oligonucleotide and target analyte mixture to the immobilization field; and (v) binding to the immobilization field. Missing selection library Including of recovering the oligonucleotide, the. [Selection figure] None |
| priorityDate | 2015-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
| Predicate | Subject |
|---|---|
| isDiscussedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/substance/SID419520973 http://rdf.ncbi.nlm.nih.gov/pubchem/protein/ACCP22629 http://rdf.ncbi.nlm.nih.gov/pubchem/compound/CID15478 |
Total number of triples: 12.