| abstract |
A method for making a recombinant polypeptide comprising: (a) (i) a cell capable of expressing said recombinant polypeptide asphosase alpha (SEQ ID NO: 1) and (ii) such expression. Providing a 100 L to 25,000 L fed-batch bioreactor comprising the culture medium, wherein the culture medium comprises about 25 μM to about 300 μM zinc; Culturing the cells under conditions suitable to express the recombinant asphostase alpha, wherein the pH of the culture medium is from about 6.7 to about 7.1, and the culture medium Wherein the zinc is added to the culture medium such that the zinc concentration is maintained at a concentration of about 25 μM to about 300 μM zinc. [Selection] Figure 53 |