| abstract |
The methods and compositions described herein surprisingly allow cells to be transiently treated with a stem cell viability enhancer before and / or after contacting the cell with one or more CRISPR / Cas9 components. To increase CRISPR / Cas-mediated gene editing in stem cells. Furthermore, this treatment surprisingly also results in increased stem cell engraftment in the target tissue of interest. The present disclosure, when used in combination with a stem cell viability enhancer, further increases gene editing frequency in stem cells, increases stem cell viability, and increases stem cell engraftment, one or more modified CRISPR / Cas9 configurations Also provides an element. |