| Predicate |
Object |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6837
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6848
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate |
2016-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2018-07-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2018518967-A |
| titleOfInvention |
Selective degradation of wild-type DNA and enrichment of mutant alleles using nucleases |
| abstract |
The present disclosure uses a nuclease that has substantially higher activity on double-stranded DNA compared to single-stranded DNA or single-stranded RNA and targets for subsequent enrichment relative to wild-type nucleic acid. Methods are provided for preparing mutant nucleic acids. The present disclosure also includes a method for enriching the target mutant nucleic acid and for preparing an unmethylated / methylated nucleic acid of interest for subsequent enrichment. This method is a novel method that results in preferential cleavage of the wild-type nucleic acid, thereby allowing subsequent enrichment of the mutated target sequence of interest. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022530483-A |
| priorityDate |
2015-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |