| abstract |
The present invention relates to a method for generating virus-free cells using nucleases for use in transplantation. The nuclease can be a CRISPR / Cas9 complex with a guide RNA to target and inactivate the viral genome in the cell. Prior to transplantation, nucleases degrade or destroy the virus in the cell. According to the present invention, cells can be treated ex vivo before delivery, so that a person does not experience viral infections derived from the cells after transplantation. Prior to delivery to the recipient, the nuclease is used in vitro to target the viral nucleic acid in the cell. |