| abstract |
The present description relates to an in vitro method for culturing hematopoietic stem cells (HSC) in the presence of hematopoietic stem cell expansion pyrimidoindole derivative agonists under mild thermal conditions (eg, between 38 ° C. and 40 ° C.). The combined use of mild heat and pyrimidoindole derivatives acts synergistically to promote CD34 + HSC expansion and / or differentiation into progenitor cells (eg, megakaryocyte precursors). This description also relates to expanded cell populations of HSCs and / or precursors in vitro and their use in therapy (eg, transplantation). |