| abstract |
The present invention relates to directional differentiation and maturation of mammalian hepatocytes such as human hepatocytes. The hepatocytes obtained according to the present invention exhibit a phenotype similar to that of the primary hepatocytes than previously shown. In particular, the present invention relates to Src kinase inhibitors, precursors, vitamin D including metabolites and analogs, hypoxia-inducing compounds, sphingosine and sphingosine derivatives, peroxisome proliferator activation of mammalian hepatocytes such as human hepatocytes It relates to exposure to at least one maturation factor selected from the group consisting of a receptor (PPAR) activator, a platelet activating factor (PAF), a PKC inhibitor, and combinations thereof. |