| abstract |
Methods are provided for linking polypeptides (including peptides and proteins) to other moieties using radical mimetic thiol-ene chemistry. For example, introducing a reactive thiol group into a polypeptide to modify it, and then reacting the thiol group with an olefin-containing reagent or alkyne-containing reagent under conditions that support a radical thiol-ene or thiol-in reaction. . A reactive thiol group is a thiol group that is more active with respect to radical thiol-ene reactions than a cysteine thiol group, such as a thiol group separated by at least two carbon atoms from a peptide backbone, such as a thiol group of a homocysteine residue. is there. Also included are compositions and biomaterials comprising the linked polypeptides, such as peptide and protein conjugates, and biocompatible hydrogel polymers based on thiol-enes, as well as their use in the medical field. [Selection] Figure 8B |