http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018505661-A
Outgoing Links
Predicate | Object |
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classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-40 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-52 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-55 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-24 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-468 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-32 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-2207 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02 |
filingDate | 2015-12-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2018505661-A |
titleOfInvention | Enzyme one-pot reaction for double polypeptide conjugation in a single step using sortase |
abstract | (I) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, where X can be any amino acid residue), the amino acid sequence LPXTA (SEQ ID NO: 31, where X is any A second polypeptide comprising 2 amino acid residues), a third polypeptide having two N-termini, wherein oligoglycine G m (m = 2 (SEQ ID NO: 22) or m = 3 (SEQ ID NO: 23) or m = 4 (SEQ ID NO: 24) or m = 5 (SEQ ID NO: 25)) having an amino acid sequence at the first N-terminus, and oligoalanine A m (m = 2 (SEQ ID NO: 26) or m = 3 (SEQ ID NO: 27) or m = 4 (SEQ ID NO: 28) or m = 5 (SEQ ID NO: 29)) the amino acid sequence at the second N-terminus A third polypeptide derived from Staphylococcus aureus sortase A, a fourth polypeptide having sortase activity, and Streptococcus pyogenes sortase A A method for making an enzymatic conjugation product of three polypeptides comprising simultaneously incubating a fifth polypeptide having sortase activity and recovering the conjugate from the reaction mixture As reported herein. |
priorityDate | 2014-12-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 1808.