http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018504898-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-682 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2015-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2018-02-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2018504898-A |
| titleOfInvention | Detection method based on signal-amplified DNA cascade reaction with target extension |
| abstract | Methods, compositions and kits for detecting a target nucleic acid in a sample. The method comprises a first signal DNA generating sequence, an endonuclease recognition site, and a sequence complementary to the 3 ′ end of the target nucleic acid in the 5 ′ to 3 ′ direction in the presence of a polymerase and an endonuclease. A second oligonucleotide comprising, in 5 ′ to 3 ′ direction, a second signal DNA generating sequence, an endonuclease recognition site, and a sequence homologous to the first signal DNA generating sequence of the first oligonucleotide In a 5 ′ to 3 ′ direction, a third oligonucleotide and sample comprising a sequence homologous to the third signal DNA generating sequence, the endonuclease recognition site, and the second signal DNA generating sequence of the second oligonucleotide. Including contacting. The reaction causes a signal cascade. The polymerase preferably has strand displacement activity, and the target nucleic acid is preferably microRNA. |
| priorityDate | 2014-12-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 130.