| abstract |
The present invention provides a dimer comprising a first polypeptide and a second polypeptide, wherein each of the first and second polypeptides comprises at least one immunoglobulin single variable domain (ISVD); A C-terminal extension comprising a cysteine moiety (preferably at the C-terminus), wherein the first polypeptide and the second polypeptide comprise the cysteine moiety of the first polypeptide and the second polypeptide With respect to dimers that are covalently linked via a disulfide bond between the cysteine portion of the protein, the dimer outperforms benchmark constructs (eg, homogeneous multivalent and multispecific constructs) in various assays. It was. The present invention provides a method for making the dimers of the present invention. |