| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_47cc435e1d443f13180f7766df104d9d |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N11-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M3-00 |
| filingDate |
2017-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f8c7599e49271c01dd77a8ca94f9365a
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ea7cc77a0f019581f55c87ea626d3d79
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_150b88977ce8bf1155edd94547cf9342
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a9d9db1752e7565adfd4596afc642535 |
| publicationDate |
2018-12-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2018201463-A |
| titleOfInvention |
Methods and kits for preparing stem cells |
| abstract |
Stem cells are cultured, and undifferentiated stem cells are selectively obtained from the cultured stem cells. The method for preparing a stem cell of the present invention comprises a step of culturing a stem cell on a substrate containing a stimulus-responsive polymer, and a buffer solution is contacted with the substrate at a temperature of less than 38 ° C. for 30 seconds to 10 minutes. And detaching the cultured stem cells from the substrate, wherein the buffer has a pH greater than 6 and less than 8, and has a Ca 2+ concentration and a Mg 2+ concentration of 1 ppm or less. [Selection figure] None |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2022080408-A1
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-2020036096-A1 |
| priorityDate |
2017-06-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |