abstract |
Disclosed are materials and methods for conjugating a water-soluble polymer to a therapeutic protein. The therapeutic protein, or biologically active thereof, under conditions that produce a reduced cysteine sulfhydryl group on the therapeutic protein and allow conjugation of a water soluble polymer to the reduced cysteine sulfhydryl group. A method of preparing a therapeutic protein complex comprising contacting a fragrance fragment with a thiol reducing agent and said water soluble polymer, or functional derivative thereof. [Selection figure] None |