http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018153097-A
Outgoing Links
Predicate | Object |
---|---|
assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_80787665b837ed3eb503bbcd27c0043a |
classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N1-02 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N7-02 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N7-02 |
filingDate | 2015-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5d48fc6df026742ac8abd33ba56e44de http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_50a139d03d14cec6799c5efb445d104e |
publicationDate | 2018-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2018153097-A |
titleOfInvention | Cell culture container, apparatus, and separation and recovery method |
abstract | The present invention provides a cell culture container, an apparatus, and a separation and recovery method that improve the quality of cells to be cultured and reduce complicated passage operations. A separation / recovery membrane 10 having a temperature-responsive portion and a pH-responsive portion, and a thin film 2 containing a pH / temperature-responsive polymer that changes in hydrophilicity 2c and hydrophobicity 2a depending on the temperature and pH is fixed to a support 1 is provided. The cell culture is performed using the used cell culture vessel, and the pH and temperature change step for changing the pH and temperature of the separation and recovery membrane 10, and the separation and recovery in which the pH and temperature are changed in the pH and temperature change step. A cell recovery step of supplying a membrane treatment solution to the surface of the membrane 10, collecting the supplied membrane treatment solution by the cell collection device, and collecting the cells 20 adsorbed to the cell binding site 2b. To separate and recover automatically. [Selection] Figure 4 |
priorityDate | 2015-07-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 46.