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filingDate 2018-03-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2018-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2018146578-A
titleOfInvention Microscope inspection system and microscope inspection method for quantifying fluorescence
abstract A microscopy system and a microscopy method for quantifying fluorescence are provided. A microscopy method for quantifying the fluorescence of protoporphyrin IX is a step of imaging an object region 7 on a first detector field 21 and a second detector field 23, each of which is wavelength-dependent. A first optical filter 33 and a second optical filter 34 having different transmission characteristics are disposed in the beam path between the object region and the detector field, respectively, and at least first and second fluorescence of protoporphyrin IX. A step of exciting, a step of recording the first and second images, and a spatially dependent fluorescence intensity of the first fluorescence in the object region by obtaining values representing the fluorescence intensities at a plurality of positions in the object region. Determining the radiation intensity of the two detector fields, the wavelength-dependent detection efficiency of the two detector fields, It is determined based on the fluorescence spectrum of the fluorescence-second fluorescence. [Selection] Figure 1
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