http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018138011-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_af92759c928daeb7f0daabbb0499f059 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate | 2017-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_44de364f9ee15b3a46b62baac88ef37d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e16eda3b6ba46a78961c48302f00f8c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_002bcf6fd1d899cb34b867780ec9bffc http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cb0c5741473bcaf1ba8fd08c328d1037 |
| publicationDate | 2018-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2018138011-A |
| titleOfInvention | Nucleic acid amplification method |
| abstract | An object of the present invention is to provide a nucleic acid amplification method that can more easily suppress false positives caused by carryover. A first nucleic acid amplification step using a target nucleic acid contained in a sample as a template, and a second nucleic acid amplification step using the amplification product generated in the first nucleic acid amplification step as a template; In the first nucleic acid amplification step, a DNA polymerase that selectively amplifies nucleic acid not containing uracil base is used to generate an amplification product not containing uracil base, and in the second nucleic acid amplification step, a primer containing uracil base and / or The above-mentioned problems are solved by a nucleic acid amplification method characterized by using dUTP and a DNA polymerase capable of amplifying a nucleic acid containing a uracil base to produce an amplification product containing a uracil base. [Selection figure] None |
| priorityDate | 2017-02-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.