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filingDate 2017-03-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bb2d1615ff6133a339fc499ecc8aa271
publicationDate 2018-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2018108071-A
titleOfInvention Method for obtaining aptamers that specifically bind to low molecular nitrogen-containing organic compounds by electrophoresis
abstract An object of the present invention is to provide a method for obtaining an aptamer that specifically binds to a low molecular nitrogen-containing organic compound from a DNA library by electrophoresis. The present invention provides a method for obtaining an aptamer that specifically binds to a low molecular nitrogen-containing organic compound, and comprises the following steps: (a) an aqueous solution containing the low molecular nitrogen-containing organic compound and a DNA library. (B) a complex containing the single-stranded DNA contained in the DNA library and the low-molecular nitrogen-containing organic compound by fractionating the aqueous solution via electrophoresis using an electroosmotic flow-suppressing capillary (C) amplifying the single-stranded DNA contained in the complex by a PCR method, and specifically, the amplified single-stranded DNA is specific to the low molecular nitrogen-containing organic compound. Obtaining as an aptamer to be bound. [Selection] Figure 2
priorityDate 2016-11-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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