http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2018036146-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_60e66be87630d7cef0ce770e1d898eec |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-88 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N30-74 |
| filingDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2ab3b21fe5cafa6f39d6611d452ccd4b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e7a75829eeba935b565779cf2bc7f46d |
| publicationDate | 2018-03-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2018036146-A |
| titleOfInvention | High-precision absolute quantification of adsorbed proteins |
| abstract | PROBLEM TO BE SOLVED: To provide a method enabling high-accuracy evaluation of protein adsorptivity to a substrate without using an expensive reagent and an expensive apparatus. A protein and a non-protein constituent amino acid are prepared by mixing a protein and a non-protein constituent amino acid to prepare a mixed sample solution. After contacting the mixed sample solution with a substrate, the protein and the non-protein constituent amino acid contained in the mixed sample solution after the contact Is hydrolyzed. Further, the calibration solution containing the amino acid mixed standard solution and the non-protein constituent amino acid is hydrolyzed. A fluorescent reagent is added to the mixed sample solution and the calibration solution after the hydrolysis treatment to quantify the amount of protein-derived amino acids in the mixed solution. At this time, the calibration solution is used for calibration of the quantitative result of amino acids. The amount of protein adsorbed on the substrate is calculated from the obtained quantitative result. [Selection] Figure 2 |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111505160-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-111505160-B |
| priorityDate | 2016-08-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 94.