| abstract |
The present invention provides a method for producing a fusion protein in which an antibody is fused with a lysosomal enzyme. A method for producing a fusion protein in which an antibody and a human lysosomal enzyme are fused, comprising: (a) culturing mammalian cells producing the fusion protein in a serum-free medium, and culturing the fusion protein in a culture solution (B) a step of recovering the culture supernatant by removing the mammalian cells from the culture solution; and (c) a substance having an affinity for the fusion protein from the culture supernatant. Using the column chromatography using the material bound to the solid phase as the stationary phase, the column chromatography using the material having affinity for the phosphate group as the stationary phase, and the size exclusion column chromatography, the fusion protein And a step of purifying the method. [Selection] Figure 1 |