| abstract |
Provided is a technique for generating a more uniform differentiated cell population from human-derived pluripotent cells. By culturing stem cell populations (such as embryonic stem cells) in a mixture of growth conditions that induce differentiation and establish neural progenitor cell populations, neural progenitor cells, differentiated neurons, glial cells and astrocytes Technology that creates groups. Technology to further differentiate progenitor cells into various different neurophenotypes including dopaminergic neurons in culture and differentiated cell populations or neural progenitor cells can be generated in large quantities for use in drug screening and treatment of neurological diseases Technology. Differentiate medium primate pluripotent stem (pPS) cells containing two or more ligands that bind to growth factor receptors, selected from antibodies to receptors for EGF, bEGF, PDGF, IGF-1 and their ligands Said cell population obtained by steps. A method of further culturing the cell population in a medium containing a cAMP activator, a neurotrophic factor, or a combination thereof. [Selection figure] None |