abstract |
A systematic approach for mutagenesis to a restriction endonuclease and a method comprising a screening assay that allows selection of the desired protein. A restriction endonuclease having at least one artificially introduced mutation and at least an overall accuracy index improvement factor of 2, from the group consisting of BamHI, NcoI, EcoRI, SalI, AgeI and NheI. A restriction endonuclease having cognate cleavage activity with the selected restriction endonuclease. The composition comprises a restriction endonuclease having reduced star activity as defined by the overall accuracy index improvement factor. [Selection figure] None |