| abstract |
Described are methods of making self-derived compositions useful for the treatment of damaged and / or injured connective tissue, chronic tendonosis, chronic muscle tears, and / or chronic degenerative arthropathy, and skin inflammatory disorders in mammals The The method includes preparing an anti-inflammatory / anti-catabolic component of the autologous composition comprising IL-1ra and TIMP. The step of preparing the anti-inflammatory / anti-catabolic component comprises the following steps: collecting blood from the mammal; sending the blood to a tube; preferably in the presence of sodium citrate, the blood is about 37 ° C. Incubating at a temperature of about 39 ° C. for about 24 hours; centrifuging the blood to separate the blood into a supernatant component and a cell fraction; and collecting the supernatant component. The method further includes the step of preparing a regenerative component of the autologous composition, the step comprising: collecting blood from a mammal; blood in the presence of about 4% citrate. Feeding the tube; centrifuging the blood to separate the platelet-rich plasma component from whole blood; collecting the platelet-rich plasma component; and mixing the supernatant component with the platelet-rich plasma component to produce the autologous composition Providing stage. Also, a method of treating damaged and / or injured connective tissue, chronic tendonosis, chronic muscle tears, and / or chronic degenerative arthropathy, and skin inflammatory disorders in a subject with an autologous composition; Autologous compositions for treating damaged and / or injured connective tissue, chronic tendonosis, chronic muscle rupture, and / or chronic degenerative arthropathy, and skin inflammatory disorders; and damaged and / or in mammals Also provided is the use of an autologous composition to treat damaged connective tissue, chronic tendonosis, chronic muscle rupture, and / or chronic degenerative arthropathy, and skin inflammatory disorders. |