abstract |
The present invention relates to a recombinant vector, a transformed cell into which the recombinant vector is introduced, a ribozyme expressed from the recombinant vector, a pharmaceutical composition for preventing or treating liver cancer containing the recombinant vector or ribozyme, and the composition The present invention relates to a method for treating hepatic cancer using sucrose. The present invention comprises a ribozyme comprising: (i) a tissue-specific promoter; and (ii) a trans-splicing ribozyme targeting a cancer-specific gene and a gene of interest linked to the 3 ′ exon of the ribozyme. A target gene expression cassette, wherein the expression cassette has a splicing donor / splicing acceptor sequence (SD / SA sequence) linked to the 5 ′ end of the ribozyme-target gene expression cassette; WPRE (Woodchuck hepatitis virus Posttranscriptional Regulatory Element) is linked to the 3 ′ end, and (iii) a nucleic acid sequence that recognizes microRNA-122a (microRNA-122a, miR-122a) is added to the 3 ′ end of the WPRE. It is connected. [Selection] Figure 1 |