abstract |
Methods are provided for performing target nucleic acid detection, probing, mapping, and target sequencing using guide RNA and Cas9 protein. A method is provided for detecting the binding of a guide RNA / Cas9 complex to a target nucleic acid, wherein the guide RNA comprises a 3 'tail sequence capable of hybridizing to a probe. A method is provided for detecting binding of a guide RNA / Cas9 complex to a target nucleic acid, wherein the complex is physically detected. |