http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017522031-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-501 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2521-319 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-149 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-107 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2565-629 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2563-159 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-162 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-307 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-125 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2533-107 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2015-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2017522031-A |
| titleOfInvention | Single nucleotide detection method |
| abstract | Methods are provided for sequencing nucleic acids such as DNA or RNA. It comprises (1) generating a single nucleoside triphosphate stream by progressive pyrophosphorolysis of nucleic acids; (2) in the presence of a polymerase and a ligase, at least one said single nucleoside triphosphate. (A) a first single-stranded oligonucleotide labeled with a characteristic detectable element in an undetectable state, and (b) a second capable of hybridizing to a complementary region of the first oligonucleotide. Generating at least one substantially double-stranded oligonucleotide spent probe by reacting with a corresponding probe system comprising a single-stranded oligonucleotide and a third single-stranded oligonucleotide; (3) Digest the used probe with an enzyme with double-stranded exonuclease activity to detect the detectable state Generating a single-stranded fourth oligonucleotide that is at least partially a sequence complement of the first oligonucleotide; (4) reacting the fourth oligonucleotide with another first oligonucleotide; Generating a substantially double-stranded oligonucleotide product corresponding to the spent probe; (5) periodically repeating steps (3) and (4); (6) step Detecting the characteristic detectable element released in each iteration of (3). Suitably, the detectable element is a fluorophore. The method of the present invention produces a stronger fluorescent signal from a single nucleoside triphosphate than previously described. A suitable probe system is also disclosed. |
| priorityDate | 2014-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 325.