| abstract |
Various aspects and embodiments of the present disclosure provide for multiple RNA regulatory strategies in mammals, including RNA triple helix structures, introns, microRNAs and ribozymes, and Cas-based CRISPR transcription factors and ribonuclease-based RNA processing in human cells. It relates to a combination method and composition. The methods and compositions of the present disclosure, in some embodiments, include proteins and proteins from transcripts expressed by a single compact RNA polymerase II for efficient regulation of synthetic constructs and endogenous human promoters. Enables multiplex production of multiple guide RNAs. |