| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-10068
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T2207-30032
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-05 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T7-254
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-44791
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-0625
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-0655
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-00009
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-0005
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-0605
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G06T7-0016
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-101
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61B1-0676 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-33
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
| filingDate |
2015-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2017-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2017505610-A |
| titleOfInvention |
Simultaneous extraction and separation of RNA and DNA from single cells using electrophoresis techniques |
| abstract |
Disclosed are devices and methods for preparing RNA and DNA from a single cell. In particular, the present invention relates to a method for simultaneously extracting RNA and DNA from a single cell by electrophoresis and separating nucleic acids. Single target cells are destroyed using an electric field so that the plasma membrane is selectively destroyed without destroying the nuclear membrane. Cytoplasmic RNA is separated from the nucleus by performing isotachophoresis (ITP) in the presence of a sieving matrix that selectively reduces the mobility of the nucleus. During ITP, cytoplasmic RNA accumulates at the ITP interface between the leading and trailing electrolytes and can later be extracted so that it does not contain nuclear DNA. The method can be performed in a microfluidic device in which all the steps of the process are fully automated. [Selection] Figure 1C |
| priorityDate |
2014-01-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |