| abstract |
The invention relates to a nucleic acid molecule comprising or consisting of a nucleic acid sequence encoding the vesicular stomatitis virus envelope glycoprotein (VSV-G) linked to a (poly) peptide comprising or consisting of a cell membrane binding domain, The nucleic acid sequence in the 5 ′ to 3 ′ direction, (a) a first sequence segment encoding an endoplasmic reticulum (ER) signal sequence; (b) comprising or consisting of a cell membrane binding domain A nucleic acid molecule comprising: a) a second sequence segment encoding a peptide; (c) a third sequence segment encoding a linker; and (d) a fourth sequence segment encoding said VSV-G. Furthermore, the present invention provides a vector comprising the nucleic acid molecule of the present invention, a host cell comprising the vector or the nucleic acid molecule, a polypeptide encoded by the nucleic acid molecule, and a method for producing a polypeptide encoded by the nucleic acid molecule. About. In addition, the present invention relates to pseudotyped lentiviral vector particles, methods for transducing cells, and kits comprising various combinations of the nucleic acid molecules, vectors, polypeptides and host cells of the present invention. |