| abstract |
A novel composition for removing impurities such as protein aggregates from a sample containing a protein of interest, eg, an antibody, is provided. Such a composition can be used during the protein purification prior to the virus filtration step to remove aggregates and protect the virus filter from soiling, thereby increasing the filter capacity of the virus. The porous solid support comprises a copolymer having at least two monomers, wherein at least one of the monomers comprises acrylamide and at least the second monomer comprises a hydrophobic binding group. The solid support selectively binds protein aggregates, thereby separating the monomeric protein of interest from the protein aggregates. This method can be performed under neutral to high pH and high conductivity conditions. |