http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2017504336-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y301-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y207-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1241 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-34 |
| filingDate | 2015-01-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2017-02-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2017504336-A |
| titleOfInvention | Cationic chelator hot start |
| abstract | The present invention is in the field of the control of enzymatic activity in nucleic acid modification reactions. The present invention describes a method for controlling enzymatic activity by the addition of chelating agents to the reaction composition, both the binding of divalent cations to these chelating agents and the pH of commonly used buffers. Take advantage of the fact that is temperature dependent. PCR experiments that are hindered by non-specific by-products can be controlled, so that the target sequence is amplified in a more specific manner. Nucleic acid modification reactions play a central role in modern biological and pharmaceutical research in both academic and industrial backgrounds. |
| priorityDate | 2014-01-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 81.