| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_c466d718e7d313e12594ca690399693a |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-727
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2533-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2501-115
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2500-98
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-35
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2317-75 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-2863
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-3092
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0696
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P37-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P35-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0606
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N11-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6881
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K31-7088 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-074 |
| filingDate |
2017-07-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bfd0c219b55ee9be35a02e5cedf66b36 |
| publicationDate |
2017-12-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2017221204-A |
| titleOfInvention |
Methods for producing pluripotent stem cells |
| abstract |
The present invention provides a method for propagating human stem cells as naive stem cells immediately after collection from an embryo or an induced pluripotent state, and a method for returning prime stem cells to a naive state and maintaining them for a long period of time. A method of inducing human naïve stem cells positive in Klf4 expression from human prime stem cells positive in Foxa2 expression or Klf4 expression is positive so that characteristics of a naive stem cell state can be obtained. A method of maintaining human naïve stem cells in a naive stem cell state in which Klf4 expression is positive. Presence of NM23 or NM23-S120G, NM23-P96S, and a mutant of NM23 that selectively forms one dimer from which the group of NM23-P96S having two or six C-terminal deletions is selected A method of culturing in the absence of a Rho kinase inhibitor and culturing in the presence of human HS27 foreskin fibroblast feeder cells on a plate coated with a monoclonal antibody that binds to a peptide of a specific amino acid sequence . [Selection figure] None |
| priorityDate |
2011-03-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |