abstract |
A method for determining the presence, absence or characteristics of an analyte in connection with nucleic acid sequencing. (A) binding a target polynucleotide to the membrane; and (b) a transmembrane pore present in the membrane such that the exonuclease digests individual nucleotides from one end of the target polynucleotide. And a detector comprising an exonuclease, interacting the target polynucleotide, (c) interacting the nucleotide with the pore, and (d) measuring the current through the pore during the interaction, A target polynucleotide comprising: determining the type of nucleotide thereby; and (e) repeating steps (b)-(d) at the same end of the target polynucleotide, thereby determining the sequence of the target polynucleotide A method of sequencing an analyte that is [Selection figure] None |