| abstract |
A cell disruption process that is not destructive to nucleic acids, has a low risk of contamination (contamination), has a reproducible quality and quantity of purified nucleic acid, and is suitable for automation. Providing equipment for this purpose. A crushing process comprising a thermoelectric cell 11 having a working surface and a base surface, the base surface being attached to a heat source / heat sink, wherein the working surface is disposed proximate to a reaction vessel 10 containing cells. Equipment for. Placing a closed tube reaction vessel 10 containing the necessary buffers, reagents and target biological cells for the device, maintaining the base surface of the thermoelectric cell at a constant temperature between the freezing and boiling temperatures of water; Applying direct current to the thermoelectric cell with the first polarity to freeze the target biological cell in the closed tube reaction vessel 10 and applying direct current to the thermoelectric cell with the second polarity opposite to the first polarity A process for disruption, comprising: thawing a target biological cell. [Selection] Figure 1 |