| abstract |
[Problem] To provide a bio-containing recombinant influenza virus for the production of a multivalent vaccine. PA virus gene segment, PB1 virus gene segment, mutant PB2 virus gene segment, HA virus gene segment, NA virus gene segment, NP virus gene segment, M (M1 and M2) virus gene segment and NS (NS1 and NS NS2) a vaccine comprising an effective amount of an isolated, stable and bio-containable multivalent influenza virus comprising 8 different gene segments, including a viral gene segment, wherein the mutant PB2 viral gene segment has a heterologous nucleotide sequence A heterologous sequence comprising 5 'and 3' integration sequences, including flanking 3 'or 5' coding and non-coding integration sequences, and not including contig sequences corresponding to sequences encoding functional PB2. The vaccine Kureochido sequence encoding a heterologous protein. [Selection] Figure 8 |