| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_116c851e729089df9a427027272fa290 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-165
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-3809
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-40 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-42
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-38
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-24
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-18
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-16
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/B01D15-16 |
| filingDate |
2017-01-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d0f6fcc879c03aee88c448fec0ed782e
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_711ad2aefdf5ec6b91c357e5d261b236
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_28daf390c3f4cb7367090dfb565045aa |
| publicationDate |
2017-06-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2017095505-A |
| titleOfInvention |
Protein purification method using Bis-Tris buffer |
| abstract |
The present invention provides a two-step chromatographic method for the small and large scale purification of proteins, particularly monoclonal antibodies, using only four buffers made from a mother solution. A method for purifying a protein from a solution comprising: (a)-passing the solution through a first chromatography column;-using a first elution buffer and the first chromatography column. Eluting the crude protein eluent from the first chromatography step; and (b)-passing the crude protein eluate obtained at the end of step (a) through a second chromatography column; -A second chromatography step comprising recovering the purified protein from the second chromatography column using a second elution buffer, wherein each buffer comprises Bistris; Method. [Selection figure] None |
| priorityDate |
2011-11-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |